One of the most widely used commercial screens, the BD GeneOhm MRSA assay (BD Diagnostics, Becton Dickinson, NJ), simultaneously detects targets in the staphylococcal cassette chromosome mec (SCC mec) and S. These offer significant advantages over culture, which takes 2 to 5 days to produce a positive result, and their use has been associated with reduced transmission of MRSA in both intensive care unit (ICU) and surgical settings ( 4, 11, 12). Screening tests have recently been introduced that detect single molecular targets specific to methicillin-resistant Staphylococcus aureus (MRSA) ( 21, 23). It also proves that commercial PCR can be performed direct on pooled samples in selective broth, without the need for overnight incubation.
This study demonstrates that the Rotor-Gene 6000 thermal cycler is a reliable platform for use with the BD GeneOhm assay. The sensitivity of the BD GeneOhm assay was increased to 100% when the results of in-house PCR and patient note review were taken into account. Sensitivities and specificities of 85.3% and 95.8% for the Rotor-Gene and 81% and 95.7% for the Smart-Cycler were obtained, compared with broth enrichment. A total of 1,428 pooled MRSA screens were tested. Samples yielding discrepant results were subjected to detailed analysis with an in-house PCR and patient note review.
Results were compared with the same assay performed on the Smart-Cycler II platform and overnight broth culture. In this study, one of the largest to date, we evaluated the performance of the BD GeneOhm MRSA assay on the Rotor-Gene 6000 thermal cycler, using samples taken directly from pooled MRSA screens. As health services move toward universal methicillin-resistant Staphylococcus aureus (MRSA) screening for hospital admissions, the most cost-effective approach is yet to be defined.
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